Sarkar et al. Figure 2
Northern blot analysis to depict the expression of MMTV and jun B in various cancerous and normal mouse tissues. Demonstration of the expression of MMTV (Panel A) and jun B
(Panel B) in the lactating mammary glands of BALB/fC3H (lane 1) and C57BLfGR (lane 2),
and non-lactating mammary gland of BALB/fC3H (lane 3), RIII (lane 4), and WLCO (lane 5) mice.
Lane 6 contained an RNA sample from a C57BLfGR tumor as control. RNA was isolated from the
tissue, fractionated by an electrophoresis gel, transferred onto a Zeta-probe membrane and hybridized with a MMTVrep probe (Panel A). Subsequently, the filter was stripped off and rehybridized with
a jun B probe (Panel B). In each case the filter was exposed to an x-ray film
overnight at -70 ° C.