FIGURE 4

Mono P HR 5/20 FPLC chromatofocusing elution profile of RBPs following fractionation by Sephadex G-75 and Superose 12 HR 10/30 FPLC (A). A pH gradient was employed using 0.025 M bis-Tris, pH 6.7 and polybuffer 74, pH 5.0. A 0.5 ml/min flow rate, 0.5 cm/min chart speed and 0.1 AUFS were used. Native-PAGE analysis of the corresponding peaks (B). Peak 1 (lane 1); peak 2 (lane 2).