Harris et al. Figure 1

FIGURE 1


A schematic of local nucleotide sequence alignments for ER DBD exon 2 and exon 3 nucleotides ranging from (898 bp to 1116 bp) of the ER cDNA (GENBANK locus HSERR) vs Vitelligenin A1 (GENBANK locus XLVITA15) nucleotides ranging from (-428 to -278) upstream from the Vitelligenin A1 gene transcription start site.


The nucleotide sequence of VITA1 showing maximal subsequence alignment with ER DBD from 1a above is shown. The EREs of VITA1 are in bold type with the DNA major groove ERE halfsites underlined. Nucleotide base pair matches are starred. Vertical lines indicate purine or pyrimidine conserved transitions. Below the ER DBD cDNA sequence is the corresponding amino acid sequence in Dayhoff (17) one-letter code with the amino acids numbered as in the human ER (18). The DNA recognition helix is underlined.


The maximally similar nucleotide subsequence of the same VITA1 sequence (-428 to -278) as above vs the ER cDNA (954 bp to 1005 bp) encoding the ER DBD DNA recognition helix is shown.


The maximally similar nucleotide subsequence of the same VITA1 sequence (-428 to -278) as above vs the ER cDNA (964 to 1023) which spans the splice junction site of ER DBD exon 2 and exon 3 encoding the ER DNA recognition helix of exon 2 and a beta strand of exon 3 is shown.


The maximally similar nucleotide subsequence of the same VITA1 sequence (-428 to -278) as above vs the ER cDNA (1120 to 1206) of exon 4 which encodes a predicted alpha helix is shown.